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Dual specificity antibodies using a double‐stranded oligonucleotide bridge
Author(s) -
Chaudri Zahida N.,
Bartlet-Jones Michael,
Panayotou George,
Klonisch Thomas,
Roitt Ivan M.,
Lund Torben,
Delves Peter J.
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00466-4
Subject(s) - oligonucleotide , antibody , chemistry , linker , microbiology and biotechnology , nucleotide , combinatorial chemistry , computational biology , biochemistry , biology , dna , genetics , gene , computer science , operating system
The covalent conjugation of oligonucleotides to antibody Fab’ fragments was optimized by using oligonucleotides modified with a hexaethylene linker arm bearing three amino groups. One oligonucleotide was coupled to antibody of one specificity and a complementary oligonucleotide to antibody of a second specificity. The antibodies were then allowed to hybridize by base pairing of the complementary nucleotide sequences and the generation of bispecific antibody was analyzed on SDS‐PAGE and confirmed using BIAcore analysis. The strategy of complementary oligonucleotide‐linked bispecific molecules is not limited to antibodies but is applicable to linking any two molecules of different characteristics.