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Suppression of the neoplastic phenotype by transfection of phospholipase C β 3 to neuroendocrine tumor cells
Author(s) -
Stålberg Peter,
Wang Shu,
Larsson Catharina,
Weber Günther,
Öberg Kjell,
Gobl Anders,
Skogseid Britt
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00457-3
Subject(s) - carcinogenesis , transfection , biology , cell culture , in vivo , in vitro , cancer research , cell growth , neuroendocrine tumors , microbiology and biotechnology , endocrinology , cancer , biochemistry , genetics
The expression of phospholipase C β 3 (PLCB3) is low or absent in several neuroendocrine neoplasias. To investigate the role of PLCB3 in the neuroendocrine tumorigenesis, we transfected a PLCB3 construct to three neuroendocrine tumor cell lines with a low PLCB3 expression. The growth rate and tumorigenicity were assessed in vitro by [ 3 H]thymidine incorporation and cell counting, in vivo, by xenografting to nude mice. In vitro, PLCB3 expressing clones showed a significant growth inhibition. The tumor weight was reduced for one of the two xenografted PLCB3 ‐transfected cell lines and in both, a reduced number of proliferating (Ki‐67 positive) cells was observed. This study implies an essential role for PLCB3 in the neuroendocrine tumorigenesis.