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Thiamine repression and pyruvate decarboxylase autoregulation independently control the expression of the Saccharomyces cerevisiae PDC5 gene
Author(s) -
Muller Elize H.,
Richards Emma J.,
Norbeck Joakim,
Byrne Kerry L.,
Karlsson Karl-Anders,
Pretorius Gert H.J.,
Meacock Peter A.,
Blomberg Anders,
Hohmann Stefan
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00449-4
Subject(s) - pyruvate decarboxylase , thiamine , saccharomyces cerevisiae , mutant , biochemistry , gene , gene expression , gene isoform , psychological repression , biology , chemistry , enzyme , alcohol dehydrogenase
The Saccharomyces cerevisiae gene PDC5 encodes the minor isoform of pyruvate decarboxylase (Pdc). In this work we show that expression of PDC5 but not that of PDC1 , which encodes the major isoform, is repressed by thiamine. Hence, under thiamine limitation both PDC1 and PDC5 are expressed. PDC5 also becomes strongly expressed in a pdc1 Δ mutant. Two‐dimensional gel electrophoresis of whole protein extracts shows that thiamine limitation stimulates the production of THI gene products and of Pdc5p. Deletion of PDC1 only stimulates production of Pdc5p. We conclude that the stimulation of PDC5 expression in a pdc1 Δ mutant is not due to a response to thiamine limitation.