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Endometrial expression of cellular protooncogene c‐ ski and its regulation by estradiol‐17β
Author(s) -
Yamanouchi Keitaro,
Soeta Chie,
Harada Rie,
Naito Kunihiko,
Tojo Hideaki
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00424-x
Subject(s) - in situ hybridization , uterus , messenger rna , estrogen , ovariectomized rat , estrous cycle , endocrinology , medicine , downregulation and upregulation , gene expression , biology , andrology , gene , biochemistry
The expression of the cellular protooncogene c‐ ski was examined in the rat uterus. In situ hybridization revealed that c‐ ski mRNA was expressed in the uterus of the adult rat on the day of estrous and localized mainly in the luminal and glandular epithelia. To test the possibility that the expression of c‐ ski mRNA is induced by estrogen, rats were ovariectomized and estradiol‐17β (E 2 ) was injected. The expression of c‐ ski mRNA was upregulated 3 h after E 2 treatment, reaching the highest level at 6 h and this persisted until 24 h; the E 2 ‐induced expression of c‐ ski mRNA was restricted to the luminal and glandular epithelia. These results suggest that the c‐ ski gene plays a role in uterine epithelial cell proliferation and mediates the proliferative action of E 2 .