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Ultraviolet‐B induction of interstitial collagenase and stromelyin‐1 occurs in human dermal fibroblasts via an autocrine interleukin‐6‐dependent loop
Author(s) -
Brenneisen Peter,
Wlaschek Meinhard,
Wenk Jutta,
Blaudschun Ralf,
Hinrichs Ralf,
Dissemond Joachim,
Krieg Thomas,
Scharffetter-Kochanek Karin
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00402-0
Subject(s) - interstitial collagenase , autocrine signalling , collagenase , matrix metalloproteinase , connective tissue , photoaging , chemistry , interleukin , microbiology and biotechnology , mmp1 , cytokine , cancer research , immunology , biology , biochemistry , pathology , gene expression , enzyme , medicine , receptor , gene , genetics
Ultraviolet‐B irradiation of human dermal fibroblasts has earlier been shown to induce matrix‐degrading metalloproteinases, thus driving connective tissue degradation in photoaging and photocarcinogenesis. Herein, we report that Ultraviolet‐B irradiation led to a dramatic increase in specific mRNA and protein levels of interstitial collagenase, stromelysin and interleukin‐6. By contrast, the major tissue inhibitor of matrix‐degrading metalloproteinases, TIMP‐1, was unaffected. Monospecific neutralizing antibodies directed against human interleukin‐6 significantly reduced the interstitial collagenase and stromelysin‐1 protein levels. Taken together, our data provide the first evidence that Ultraviolet‐B induction of interstitial collagenase and stromelysin‐1 occurs via the synthesis and release of interleukin‐6. Hence, this newly identified autocrine mechanism may contribute to dermal photodamage.