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The auxin‐binding protein Nt‐ERabp1 alone activates an auxin‐like transduction pathway
Author(s) -
Leblanc N,
Perrot-Rechenmann C,
Barbier-Brygoo H
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00398-1
Subject(s) - auxin , microbiology and biotechnology , peptide , transduction (biophysics) , chemistry , hyperpolarization (physics) , signal transduction , biochemistry , biophysics , biology , gene , stereochemistry , nuclear magnetic resonance spectroscopy
Hyperpolarization of tobacco protoplasts is amongst the earliest auxin responses described. It has been proposed that the auxin‐binding protein, ABP1, or a related protein could be involved in the first step of auxin perception at the plasma membrane. Using for the first time homologous conditions for interaction between the protein Nt‐ERabp1 or a synthetic peptide corresponding to the C‐terminus and tobacco protoplasts, we have demonstrated that both can induce the hyperpolarization response. The results show that Nt‐ERabp1 or the C‐terminal peptide alone activates the auxin pathway from the outer face of the plasma membrane.