Premium
Zinc is required in pyrrolidine dithiocarbamate inhibition of NF‐κB activation
Author(s) -
Kim Chul Hoon,
Kim Joo Hee,
Hsu Chung Y.,
Ahn Young Soo
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00390-7
Subject(s) - pyrrolidine dithiocarbamate , chemistry , zinc , nf κb , basal (medicine) , dithiocarbamate , pyrrolidine , nfkb1 , biochemistry , pharmacology , stereochemistry , medicine , biology , signal transduction , transcription factor , organic chemistry , insulin , gene
Pyrrolidine dithiocarbamate (PDTC) is a potent inhibitor of nuclear factor kappa B (NF‐κB) activation. PDTC inhibited basal NF‐κB activity of endothelial cells. PDTC, however, failed to inhibit basal NF‐κB activity after withdrawal of serum in the media, and the inhibitory effect of PDTC could be restored by addition of zinc. When various preparations of metal ion‐EDTA were tested with PDTC in serum‐containing media, only Zn‐EDTA failed to block the inhibitory effect of PDTC. The dependence on zinc was also noted in PDTC inhibition of NF‐κB stimulated by TNFα. These facts suggest that zinc is required for PDTC inhibition of NF‐κB activation.