Premium
Apoptosis induced by growth factor withdrawal in fibroblasts overproducing fructose 2,6‐bisphosphate
Author(s) -
Durante Paula,
Gueuning Marie-Agnès,
Darville Martine I,
Hue Louis,
Rousseau Guy G
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00387-7
Subject(s) - fructose 2,6 bisphosphate , fructose , glycolysis , apoptosis , biochemistry , cell culture , kinase , biology , microbiology and biotechnology , chemistry , phosphofructokinase , enzyme , genetics
Fructose 2,6‐bisphosphate is a potent endogenous stimulator of glycolysis. A high aerobic glycolytic rate often correlates with increased cell proliferation. To investigate this relationship, we have produced clonal cell lines of Rat‐1 fibroblasts that stably express transgenes coding for 6‐phosphofructo‐2‐kinase, which catalyzes the synthesis of fructose 2,6‐bisphosphate, or for fructose 2,6‐bisphosphatase, which catalyzes its degradation. While serum deprivation in culture reduced the growth rate of control cells, it caused apoptosis in cells overproducing fructose 2,6‐bisphosphate. Apoptosis was inhibited by 5‐amino‐4‐imidazolecarboxamide riboside, suggesting that 5′‐AMP‐activated protein kinase interferes with this phenomenon.