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Co‐transcription of Rhizobium meliloti lysyl‐tRNA synthetase and glutamyl‐tRNA synthetase genes
Author(s) -
Pelchat Martin,
Gag Yves,
Laberge Serge,
Lapointe Jacques
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00385-3
Subject(s) - transfer rna , gene , biology , open reading frame , transcription (linguistics) , rhizobium , biochemistry , microbiology and biotechnology , escherichia coli , genetics , peptide sequence , rna , linguistics , philosophy
An open reading frame encoding a putative polypeptide very similar to several lysyl‐tRNA synthetases was found 10 nucleotides downstream of Rhizobium meliloti gltX encoding glutamyl‐tRNA synthetase. Expression of this gene complemented a mutation in lysS of Escherichia coli and led to the overexpression of a polypeptide of the expected mass (62 kDa), thus confirming that it encodes R. meliloti lysyl‐tRNA synthetase. Reverse transcription/polymerase chain reaction was used to demonstrate that this lysS gene is co‐transcribed with gltX in R. meliloti . This is the first reported case of two immediately adjacent and co‐transcribed genes encoding aminoacyl‐tRNA synthetases.