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Clusterin (SGP‐2) gene expression is cell cycle dependent in normal human dermal fibroblasts
Author(s) -
Bettuzzi S,
Astancolle S,
Guidetti G,
Moretti M,
Tiozzo R,
Corti A
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00375-0
Subject(s) - clusterin , messenger rna , gene expression , cell cycle , microbiology and biotechnology , biology , gene , cell , chemistry , apoptosis , biochemistry
In confluent human dermal fibroblasts brought to quiescence (G 0 ) by serum starvation, the S phase peaked at 24 h after serum re‐addiction and G 2 /M phase peaked at 36 h. This was confirmed by titration of h‐gas1 mRNA (a marker of G 0 phase) and histone H3 (a marker of S phase). Clusterin mRNA accumulation progressively increased in cells proceeding to confluence after seeding and to quiescence upon serum starvation, and peaked at around G 0 , in parallel with h‐gas1 mRNA. At 6 h (roughly G 1 phase) clusterin transcript formed a second peak, followed by a gradual decrease until 36 h. Correspondence of clusterin protein accumulation to its mRNA occurred solely with regard to the G 0 peak but not to the second one. The possible meaning of the cell cycle related clusterin gene expression is discussed.