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The induction of GSH synthesis by nanomolar concentrations of NO in endothelial cells: a role for γ‐glutamylcysteine synthetase and γ‐glutamyl transpeptidase
Author(s) -
Moellering Douglas,
Mc Andrew Joanne,
Patel Rakesh P,
Forman Henry Jay,
Mulcahy R.Timothy,
Jo Hanjoong,
Darley-Usmar Victor M
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00371-3
Subject(s) - nitric oxide , glutathione , antioxidant , chemistry , oxidative stress , biochemistry , radical , enzyme , nitric oxide synthase type iii , nitric oxide synthase , s nitrosoglutathione , microbiology and biotechnology , biology , enos , organic chemistry
Nitric oxide protects cells from oxidative stress through a number of direct scavenging reactions with free radicals but the effects of nitric oxide on the regulation of antioxidant enzymes are only now emerging. Using bovine aortic endothelial cells as a model, we show that nitric oxide, at physiological rates of production (1–3 nM/s), is capable of inducing the synthesis of glutathione through a mechanism involving γ‐glutamylcysteine synthetase and γ‐glutamyl transpeptidase. This novel nitric oxide signalling pathway is cGMP‐independent and we hypothesize that it makes an important contribution to the anti‐atherosclerotic and antioxidant properties of nitric oxide.