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Molecular cloning and characterization of human AOS1 and UBA2, components of the sentrin‐activating enzyme complex
Author(s) -
Gong Limin,
Li Bing,
Millas Stefanos,
Yeh Edward T.H.
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00367-1
Subject(s) - cloning (programming) , molecular cloning , enzyme , biochemistry , chemistry , characterization (materials science) , biology , gene , peptide sequence , nanotechnology , materials science , computer science , programming language
Sentrin‐1/SUMO‐1 is a novel ubiquitin‐like protein, which can covalently modify a limited number of cellular proteins. Here we report the identification of the sentrin‐activating enzyme complex, which consists of two proteins AOS1 and UBA2. Human AOS1 is homologous to the N‐terminal half of E 1, whereas human UBA2 is homologous to the C‐terminal half of E 1. The human UBA2 gene is located on chromosome 19q12. Human UBA2 could form a β‐mercaptoethanol‐sensitive conjugate with members of the sentrin family, but not with ubiquitin of NEDD8, in the presence of AOS1. Identification of human UBA2 and AOS1 should allow a more detailed analysis of the enzymology of the activation of ubiquitin‐like proteins.