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The RAMP2/CRLR complex is a functional adrenomedullin receptor in human endothelial and vascular smooth muscle cells
Author(s) -
Kamitani Shigeki,
Asakawa Makoto,
Shimekake Yoshiyuki,
Kuwasako Kenji,
Nakahara Koichiro,
Sakata Tsuneaki
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00358-0
Subject(s) - adrenomedullin , calcitonin receptor , receptor , enzyme linked receptor , interleukin 21 receptor , estrogen related receptor alpha , vascular smooth muscle , endocrinology , medicine , estrogen related receptor gamma , 5 ht5a receptor , microbiology and biotechnology , calcitonin gene related peptide , protease activated receptor 2 , biology , chemistry , biochemistry , nuclear receptor , smooth muscle , estrogen receptor , neuropeptide , cancer , breast cancer , gene , transcription factor
Adrenomedullin, a potently hypotensive peptide isolated from human pheochromocytoma, is known to elicit a rise in cAMP levels within mammalian endothelial and smooth muscle cells. Until now, however, little has been known about the adrenomedullin receptor. Recently, a group called receptor activity‐modifying proteins that complex with the calcitonin receptor‐like receptor, and thereby regulate its transport and ligand specificity, were identified. Here we show that mRNA for both the calcitonin receptor‐like receptor and the receptor activity‐modifying protein 2, but not the receptor activity‐modifying protein 1 or receptor activity‐modifying protein 3, are expressed in human endothelial and vascular smooth muscle cells. We also found that adrenomedullin increased cAMP levels in HeLa EBNA and 293 EBNA cells, expressing both the receptor activity‐modifying protein 2 and calcitonin receptor‐like receptor proteins. Thus, the receptor activity‐modifying protein 2/calcitonin receptor‐like receptor complex apparently serves as a functional adrenomedullin receptor in human endothelial and vascular smooth muscle cells.