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Mutation S543N in the thumb subdomain of the Taq DNA polymerase large fragment suppresses pausing associated with the template structure
Author(s) -
Ignatov K.B.,
Bashirova A.A.,
Miroshnikov A.I.,
Kramarov V.M.
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00353-1
Subject(s) - dna clamp , dna polymerase , polymerase , taq polymerase , microbiology and biotechnology , hot start pcr , dna polymerase ii , dna , biology , polymerase chain reaction , multiple displacement amplification , thermus aquaticus , chemistry , genetics , nested polymerase chain reaction , gene , reverse transcriptase , dna extraction
Substitution of Asn for the conserved Ser 543 in the thumb subdomain of the Taq DNA polymerase large fragment ( Klentaq DNA polymerase) prevents pausing during DNA synthesis and allows the enzyme to circumvent template regions with a complex structure. The mutant enzyme ( KlentaqN DNA polymerase) provides specific PCR amplification and sequencing of difficult templates, e.g. those with a high GC% content or strong secondary structure.