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Apoptosis of mouse liver nuclei induced in the cytosol of carrot cells
Author(s) -
Zhao Yun,
Jiang Zheng-Fan,
Sun Ying-Li,
Zhai Zhong-He
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00352-x
Subject(s) - apoptosis , dna fragmentation , microbiology and biotechnology , chromatin , in situ nick end labeling , apoptotic dna fragmentation , dna , biology , fragmentation (computing) , cytosol , terminal deoxynucleotidyl transferase , tunel assay , nuclear dna , prophase , cytochrome c , gel electrophoresis , programmed cell death , biochemistry , mitochondrial dna , enzyme , ecology , meiosis , gene
We report here the apoptosis of mouse liver nuclei induced in the cytosol of carrot cells by cytochrome c . Several typical characteristics of apoptosis, such as chromatin condensation, margination and apoptotic bodies, were detected. The result of DNA gel electrophoresis showed that DNA was degraded into nucleosomal fragments. The terminal deoxynucleotidyl transferase‐mediated dUTP‐digoxigenin nick end labelling procedure was also performed to detect the breakage of 3′‐OH ends of a DNA strand. Furthermore, we found that nuclear lamins were degraded from 88 kDa and 66 kDa to 37 kDa and 47 kDa fragments. The DNA fragmentation could be inhibited by AC‐DEVD‐CHO and AC‐YVAD‐CHO. The results indicate that the apoptosis in plant cells may share some similar pathways to apoptosis in animal cells.