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Isolation of cDNA clones encoding two isoforms of nucleoside diphosphate kinase from bovine retina
Author(s) -
Karaschuk Galina,
Kakuev Dmitry
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00349-x
Subject(s) - complementary dna , microbiology and biotechnology , nucleoside diphosphate kinase , gene isoform , biology , biochemistry , open reading frame , nucleic acid sequence , map3k7 , peptide sequence , kinase , protein kinase a , mitogen activated protein kinase kinase , gene
The cDNA encoding bovine retinal isoforms of nucleoside diphosphate kinase (NDP‐kinase, EC 2.7.4.6) has been cloned and sequenced. Based on the partial amino acid sequence of the enzyme determined after trypsin digestion of purified NDP‐kinase, primers were synthesized and used to isolate two different cDNA clones encoding the full length of two NDP‐kinase isoforms. The nucleotide sequences of these clones contained open reading frames encoding 152‐residue polypeptides with calculated molecular masses of 17.262 and 17.299 kDa, similar to that determined for the subunits of purified enzyme (17.5 and 18.5 kDa). The deduced NDP‐kinase sequences showed high similarity with the known NDP‐kinase sequences from other sources.