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Novel BNIP1 variants and their interaction with BCL2 family members
Author(s) -
Zhang Hong,
Heim Jutta,
Meyhack Bernd
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00335-x
Subject(s) - genetics , gene , splice , biology , function (biology) , computational biology , mechanism (biology) , protein family , philosophy , epistemology
By PCR and EST database searches we have identified three novel BNIP1 splice variants, and found that one of them, BNIP1‐b, contains a highly conserved BH3 domain. The BNIP1 gene has been assigned to chromosome 5q33–34. Using in vitro protein‐protein interaction assays, all BNIP1 variants were shown to interact with BCL2 and also with BCL2L1 (previously Bcl‐xL). These interactions are BH3‐independent. Furthermore, the BNIP1 variants cannot interact with BAX. The results suggest that the BNIP1 variants are novel members of the BCL2 family but function through a mechanism different from other BH3‐only members.