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Differential potency of two crosslinking plant lectins to induce formation of haptenic‐sugar‐resistant aggregates of rat thymocytes by post‐binding signaling
Author(s) -
Timoshenko A.V.,
Gorudko I.V.,
Cherenkevich S.N.,
Gabius H.-J.
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00329-4
Subject(s) - concanavalin a , diacylglycerol kinase , lectin , biochemistry , chemistry , fusicoccin , protein kinase a , kinase , trifluoperazine , protein kinase c , calmodulin , biology , enzyme , in vitro , atpase
To evaluate the significance of post‐binding events for stable aggregate formation, the aggregation/dissociation of rat thymocytes initiated by two crosslinking plant lectins, namely concanavalin A (Con A) and Solanum tuberosum agglutinin (STA), were comparatively studied. Despite intimate cell contacts in the aggregates only Con A led to establishment of haptenic‐sugar‐resistant (HSR) complexes. The presence of inhibitor II of diacylglycerol kinase, a dual calmodulin antagonist/protein kinase C inhibitor (trifluoperazine), and a sulfhydryl group reagent ( N ‐ethylmaleimide) impaired this process. The obtained results indicate that the formation of HSR cellular contacts is not an automatic response to lectin‐dependent cell association. In contrast to STA, Con A binding elicits this reaction with involvement of diacylglycerol kinase, protein kinase C and/or calmodulin as well as thiol level perturbation, as inferred by the application of target‐selective inhibitors.