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Fra‐1: a novel target for retinoid action
Author(s) -
Kaiser Astrid,
Brembeck Felix H.,
v. Marschall Zofia,
Riecken Ernst-Otto,
Wiedenmann Bertram,
Rosewicz Stefan
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00326-9
Subject(s) - junb , transactivation , activator (genetics) , retinoid , microbiology and biotechnology , reporter gene , transfection , biology , complementary dna , chemistry , gene , transcription factor , gene expression , retinoic acid , biochemistry
Using a cDNA gene expression array system, we identified fra‐1 as a novel target molecule for retinoid action in the human pancreatic carcinoma cell line Dan‐G. Retinoid treatment resulted in a significant and time‐dependent induction of the fra‐1 expression on the post‐transcriptional level. Supershift assays revealed that fra‐1 participates in the activator protein 1 complex together with c‐fos, c‐jun and junB. Transient transfection experiments using a reporter plasmid containing an activator protein site upstream of the CAT reporter gene revealed that the phorbolester‐induced CAT activity was suppressed by retinoids. Since fra‐1 lacks a transactivation function, we therefore suggest that the retinoid‐mediated induction of fra‐1 might function as a negative regulator of the activator protein 1 activity in human pancreatic carcinoma cells.