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Involvement of a chloroplast homologue of the signal recognition particle receptor protein, FtsY, in protein targeting to thylakoids
Author(s) -
Kogata Naoko,
Nishio Kazuaki,
Hirohashi Toshiya,
Kikuchi Shingo,
Nakai Masato
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00305-1
Subject(s) - thylakoid , chloroplast , signal recognition particle , arabidopsis , signal recognition particle receptor , protein targeting , biology , signal peptide , transit peptide , biochemistry , arabidopsis thaliana , membrane protein , microbiology and biotechnology , peptide sequence , plastid , gene , membrane , mutant
We isolated an Arabidopsis thaliana cDNA whose translated product shows sequence similarity to the FtsY, a bacterial homologue of SRP receptor protein. The Arabidopsis FtsY homologue contains a typical chloroplast transit peptide. The in vitro‐synthesized 37 kDa FtsY homologue was imported into chloroplasts, and the processed 32 kDa polypeptide bound peripherally on the outer surface of thylakoids. Antibodies raised against the FtsY homologue also reacted with a thylakoid‐bound 32 kDa protein. The antibodies inhibited the cpSRP‐dependent insertion of the light‐harvesting chlorophyll a / b ‐binding protein into thylakoid membranes suggesting that the chloroplast FtsY homologue is involved in the cpSRP‐dependent protein targeting to the thylakoid membranes.