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Hypothetical protein KIAA0079 is a mammalian homologue of yeast Sec24p
Author(s) -
Tani Katsuko,
Oyama Yuki,
Hatsuzawa Kiyotaka,
Tagaya Mitsuo
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00303-8
Subject(s) - endoplasmic reticulum , yeast , copii , saccharomyces cerevisiae , microbiology and biotechnology , biochemistry , biology , secretory pathway , secretory protein , vesicular transport proteins , chemistry , golgi apparatus , gene , vacuolar protein sorting
The Sec23p‐Sec24p complex is a component of COPII‐coated vesicles that mediate protein transport from the endoplasmic reticulum in yeast. The mammalian hypothetical protein KIAA0079 (KIAA0079p) exhibits sequence similarity to yeast Sec24p. KIAA0079p was co‐eluted with mammalian Sec23p on gel filtration. In vitro binding experiments revealed that the C‐terminal region of KIAA0079p binds to the N‐terminal region of mammalian Sec23p. Overexpression of KIAA0079p caused a defect in protein export from the endoplasmic reticulum. These results support the idea that KIAA0079p is a functional homologue of yeast Sec24p.