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The hypothalamic satiety peptide CART is expressed in anorectic and non‐anorectic pancreatic islet tumors and in the normal islet of Langerhans
Author(s) -
Jensen Per B,
Kristensen Peter,
Clausen Jes T,
Judge Martin E,
Hastrup Sven,
Thim Lars,
Wulff Birgitte S,
Foged Christian,
Jensen Jan,
Holst Jens J,
Madsen Ole D
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00291-4
Subject(s) - anorectic , islet , cart , endocrinology , medicine , post prandial , chemistry , food intake , insulin , diabetes mellitus , mechanical engineering , engineering
The hypothalamic satiety peptide CART (cocaine and amphetamine regulated transcript) is expressed at high levels in anorectic rat glucagonomas but not in hypoglycemic insulinomas. However, a non‐anorectic metastasis derived from the glucagonoma retained high CART expression levels and produced circulating CART levels comparable to that of the anorectic tumors. Moreover, distinct glucagonoma lines derived by stable HES‐1 transfection of the insulinoma caused severe anorexia but retained low circulating levels of CART comparable to that of insulinoma bearing or control rats. Islet tumor associated anorexia and circulating CART levels are thus not correlated, and in line with this peripheral administration of CART (5–50 mg/kg) produced no effect on feeding behavior. In the rat two alternatively spliced forms of CART mRNA exist and quantitative PCR revealed expression of both forms in the hypothalamus, in the different islet tumors, and in the islets of Langerhans. Immunocytochemistry as well as in situ hybridization localized CART expression to the somatostatin producing islet D cell. A potential endocrine/paracrine role of islet CART remains to be clarified.