Premium
Chloroplast class I and class II aldolases are bifunctional for fructose‐1,6‐biphosphate and sedoheptulose‐1,7‐biphosphate cleavage in the Calvin cycle
Author(s) -
Flechner Anke,
Gross Wolfgang,
Martin William F,
Schnarrenberger Claus
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00285-9
Subject(s) - phosphofructokinase 2 , biochemistry , fructose , cytosol , chloroplast , enzyme , biology , chemistry , bifunctional , gene , catalysis
Class I and class II aldolases are products of two evolutionary non‐related gene families. The cytosol and chloroplast enzymes of higher plants are of the class I type, the latter being bifunctional for fructose‐1,6‐ and sedoheptulose‐1,7‐P 2 in the Calvin cycle. Recently, class II aldolases were detected for the cytosol and chloroplasts of the lower alga Cyanophora paradoxa . The respective chloroplast enzyme has been shown here to be also bifunctional for fructose‐1,6‐ and sedoheptulose‐1,7‐P 2 . Kinetics, also including fructose‐1‐P, were determined for all these enzymes. Apparently, aldolases are multifunctional enzymes, irrespective of their class I or class II type.