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DNA triple helix stabilisation by a naphthylquinoline dimer
Author(s) -
Keppler Melanie,
Zegrocka Oliwia,
Strekowski Lucjan,
Fox Keith R
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00284-7
Subject(s) - footprinting , dimer , triple helix , oligonucleotide , duplex (building) , chemistry , dna , intercalation (chemistry) , dna footprinting , intermolecular force , stereochemistry , ligand (biochemistry) , helix (gastropod) , a dna , biophysics , crystallography , base sequence , biochemistry , dna binding protein , biology , gene , molecule , receptor , organic chemistry , transcription factor , ecology , snail
We have used DNase I footprinting to examine the effect of a novel naphthylquinoline dimer, designed as a triplex‐specific bis‐intercalator, on the stability of intermolecular DNA triplexes. We find that this compound efficiently promotes triplex formation between the 9‐mer oligonucleotide 5′‐CTT and its oligopurine duplex target at concentrations as low as 0.1 μM, enhancing the triplex stability by at least 1000‐fold. This compound, which is the first reported example of a triplex bis‐intercalator, is about 30 times more potent than the simple monofunctional ligand.