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PPARγ activators down‐regulate the expression of PPARγ in 3T3‐L1 adipocytes
Author(s) -
Camp Heidi S,
Whitton Amy L,
Tafuri Sherrie R
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00268-9
Subject(s) - troglitazone , adipocyte , 3t3 l1 , peroxisome proliferator activated receptor , chemistry , transcription factor , medicine , endocrinology , adipogenesis , receptor , microbiology and biotechnology , biology , biochemistry , gene , adipose tissue
Transcriptional activation of PPARγ by the anti‐diabetic compound troglitazone enhances the rate of 3T3‐L1 adipocyte differentiation. In this study, we examined the effects of troglitazone, a specific PPARγ ligand, on the expression of PPARγ during and after 3T3‐L1 adipocyte differentiation. Troglitazone treatment caused a significant decrease in PPARγ proteins and DNA binding activity. This reduction was associated with a similar decrease in transcription of PPARγ mRNA. These data suggest that in 3T3‐L1 cells, the expression of PPARγ is auto‐regulated.