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Delineation of genomic deletion in cardiomyopathic hamster
Author(s) -
Sakamoto Aiji,
Abe Makoto,
Masaki Tomoh
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00267-7
Subject(s) - hamster , biology , microbiology and biotechnology , genetics , genomic dna , mesocricetus , exon , gene
Cardiomyopathic hamster is a representative animal model for autosomal recessive cardiomyopathy. We have previously shown that the transcript of δ‐sarcoglycan is missing in the heart of cardiomyopathic hamster due to genomic deletion. Here we define the normal genomic region deleted in cardiomyopathic hamster, which spans about 30 kb interval and includes the two first exons of the δ‐sarcoglycan gene. RNA blot analysis using genomic DNA fragments covering the entire deletion as probes failed to detect any transcript other than δ‐sarcoglycan in normal hamster heart, suggesting that δ‐sarcoglycan is the only transcript defective in the heart of cardiomyopathic hamster.