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Imaging fluorescence resonance energy transfer between two green fluorescent proteins in living yeast
Author(s) -
Sagot Isabelle,
Bonneu Marc,
Balguerie Axelle,
Aigle Michel
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00258-6
Subject(s) - green fluorescent protein , förster resonance energy transfer , fusion protein , mutant , fluorescence , yeast , fluorescence microscope , protease , chemistry , bimolecular fluorescence complementation , biophysics , cleavage (geology) , microbiology and biotechnology , biology , biochemistry , enzyme , recombinant dna , gene , physics , quantum mechanics , paleontology , fracture (geology)
We show that fluorescence resonance energy transfer between two mutants of the green fluorescent protein (GFP) can be monitored by imaging microscopy in living yeast. This work is based on the constitutive expression of a GFP‐containing fusion protein and the inducible expression of the tobacco etch virus (TEV) protease. In the fusion protein, the P4.3 GFP mutant is linked to the YS65T GFP mutant by a spacer bearing the TEV protease‐specific cleavage site.