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Interaction of heparin with annexin V
Author(s) -
Capila Ishan,
VanderNoot Victoria A,
Mealy Tanya R,
Seaton Barbara A,
Linhardt Robert J
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00245-8
Subject(s) - isothermal titration calorimetry , chemistry , heparin , oligosaccharide , annexin a2 , annexin , surface plasmon resonance , heparan sulfate , phospholipid , titration , binding site , biochemistry , chromatography , membrane , inorganic chemistry , materials science , nanoparticle , cell , nanotechnology
The energetics and kinetics of the interaction of heparin with the Ca +2 and phospholipid binding protein annexin V, was examined and the minimum oligosaccharide sequence within heparin that binds annexin V was identified. Affinity chromatography studies confirmed the Ca +2 dependence of this binding interaction. Analysis of the data obtained from surface plasmon resonance afforded a K d of ∼21 nM for the interaction of annexin V with end‐chain immobilized heparin and a K d of ∼49 nM for the interaction with end‐chain immobilized heparan sulfate. Isothermal titration calorimetry showed the minimum annexin V binding oligosaccharide sequence within heparin corresponds to an octasaccharide sequence. The K d of a heparin octasaccharide binding to annexin V was ∼1 μM with a binding stoichiometry of 1:1.