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Molecular cloning of rat GADD45γ, gene induction and its role during neuronal cell death
Author(s) -
Kojima Satoshi,
Mayumi-Matsuda Keiko,
Suzuki Harukazu,
Sakata Tsuneaki
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00234-3
Subject(s) - programmed cell death , gadd45 , biology , microbiology and biotechnology , gene , neurotrophic factors , cell , apoptosis , genetics , cell cycle , receptor , cell cycle checkpoint
To study the molecular mechanism of neuronal cell death, we carried out the screening of genes which were induced during the neuronal cell death of neuronal PC12. We cloned the cDNA of rat GADD45γ, the third member of the GADD45 family. Induction of GADD45γ mRNA was observed in the neuronal cell death caused by depletion of neurotrophic factor and Ca 2+ ionophore treatment. Overexpression of GADD45γ in superior cervical ganglion neurons caused cell death. These results suggest that GADD45γ plays an important role in neuronal cell death.