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Demonstration of the pathogenic effect of point mutated keratin 9 in vivo
Author(s) -
Kobayashi Setsu,
Kore-eda Satoshi,
Tanaka Toshihiro
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00233-1
Subject(s) - keratin , in vivo , complementary dna , microbiology and biotechnology , keratin 6a , point mutation , biology , transfection , keratin 5 , keratin 8 , mutation , chemistry , gene , intermediate filament , genetics , cell , cytoskeleton
A wild type keratin 9 (K9) cDNA and a point mutated keratin 9 cDNA were injected subcutaneously into mouse skin. The hemagglutinin tag staining of the wild type K9 cDNA injected specimens mainly showed a homogeneous pattern, whereas the point mutated K9 cDNA injected specimens mainly showed a granular pattern in the suprabasal cells. Double staining of K9 and the endogenous keratin revealed the incorporation of de novo synthesized K9 into the keratin network. These results demonstrate that (1) a naked DNA transfection into mouse skin can detect the pathogenic changes of point mutated keratin in vivo and (2) the keratin 9 mutation disrupts the keratin network formation in the suprabasal cells in vivo.