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Surface display of functional fibronectin‐binding domains on Staphylococcus carnosus
Author(s) -
Liljeqvist Sissela,
Cano François,
Nguyen Thien Ngoc,
Uhlén Mathias,
Robert Alain,
Ståhl Stefan
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00232-x
Subject(s) - fibronectin , chemistry , biophysics , surface (topology) , staphylococcus , staphylococcus aureus , microbiology and biotechnology , biochemistry , biology , bacteria , genetics , mathematics , geometry , extracellular matrix
The surface expression in Staphylococcus carnosus of three different fibronectin binding domains (FNBDs), derived from fibronectin binding proteins of Streptococcus dysgalactiae and Staphylococcus aureus , has been investigated. Surface localization of the chimeric proteins containing the FNBDs was demonstrated. All three surface‐displayed FNBDs were demonstrated to bind fibronectin in whole‐cell enzyme‐linked binding assays. Furthermore, for one of the constructs, intranasal immunizations with the recombinant bacteria resulted in improved antibody responses to a model immunogen present within the chimeric surface proteins. The implications of the results for the design of live bacterial vaccine delivery systems are discussed.