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TM7XN1, a novel human EGF‐TM7‐like cDNA, detected with mRNA differential display using human melanoma cell lines with different metastatic potential 1
Author(s) -
Zendman Albert J.W,
Cornelissen Ine M.H.A,
Weidle Ulrich H,
Ruiter Dirk J,
van Muijen Goos N.P
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00230-6
Subject(s) - complementary dna , subfamily , northern blot , biology , microbiology and biotechnology , transmembrane protein , cell culture , extracellular , messenger rna , transmembrane domain , melanoma , peptide sequence , gene , receptor , cancer research , genetics
We have identified a novel 3845 bp cDNA differentially expressed in a human melanoma metastasis model. Northern blot analysis showed expression in the poorly and intermediately metastasizing cell lines and a marked downregulation in the highly metastatic cell lines. Using RT‐PCR expression was also seen in several other tumor cell lines and normal cell types of human origin. cDNA sequence analysis revealed an ORF of 687 amino acids containing seven putative transmembrane domains C‐terminally and a long N‐terminus. The gene was mapped to 16q13. Highest homology was observed with members of the EGF‐TM7 subfamily of the secretin/calcitonin receptor family. We propose the delineation of a subfamily of TM7 proteins, LN‐TM7, containing seven transmembrane proteins with a long N‐terminal extracellular part.