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The chum salmon IGF‐II gene promoter is activated by hepatocyte nuclear factor 3β
Author(s) -
Palamarchuk Alexey Y.,
Kavsan Vadim M.,
Sussenbach John S.,
Holthuizen P.Elly
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00219-7
Subject(s) - hepatocyte nuclear factor 4 , hepatocyte nuclear factors , transcription factor , activator (genetics) , biology , gene , microbiology and biotechnology , electrophoretic mobility shift assay , hepatocyte , transfection , genetics , nuclear receptor , in vitro
IGF‐II plays an important role in growth and development of vertebrates and is highly expressed in adult salmon liver. In the present study, we demonstrate that a liver‐enriched transcription factor, hepatocyte nuclear factor 3β (HNF‐3β), is an activator of the chum salmon IGF‐II gene. Multiple binding sites for HNF‐3β were identified within the 5′‐UTR using electrophoretic mobility shift assays and mutation of these sites prevents binding of HNF‐3β. In transient transfection assays it was shown that mutation of the HNF‐3β binding sites results in a substantial decrease of HNF‐3β‐activated salmon IGF‐II gene expression. This is the first identified transcription factor that is functionally involved in the regulation of fish IGF‐II expression.