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Central role of epidermal growth factor (EGF) receptor density in anchorage‐independent growth of normal rat kidney cells
Author(s) -
Lahaye Daniëlle H.T.P.,
Camps Marcel G.M.,
Van Zoelen Everardus J.J.
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00216-1
Subject(s) - epidermal growth factor , heparin binding egf like growth factor , receptor , endocrinology , downregulation and upregulation , medicine , microbiology and biotechnology , biology , epidermal growth factor receptor , transformation (genetics) , cell growth , growth factor , chemistry , biochemistry , gene
Epidermal growth factor (EGF) receptor levels are known to play a central role in density dependent growth regulation of normal rat kidney (NRK) fibroblasts. Here we show that EGF receptor expression is strongly decreased when NRK cells are cultured under anchorage independent conditions, and that expression is returned to original levels upon cell re‐adherence. Agents that stimulate anchorage independent growth (AIG) of NRK cells in the presence of EGF are shown to upregulate both EGF receptor promoter activity and 125 I‐EGF binding capacity. These data show that two aspects of phenotypic transformation of NRK cells, namely density arrest and AIG, can both directly be correlated to EGF receptor levels.