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Characterization of a cDNA encoding a precursor of Carassius RFamide, structurally related to a mammalian prolactin‐releasing peptide
Author(s) -
Satake Honoo,
Minakata Hiroyuki,
Wang Xiaoyan,
Fujimoto Masaaki
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00215-x
Subject(s) - complementary dna , crucian carp , amino acid , biology , peptide sequence , peptide , prolactin , microbiology and biotechnology , biochemistry , gene , hormone , fishery , fish <actinopterygii>
We have characterized the cDNA encoding Carassius RFamide (C‐RFa), which is structurally related to mammalian prolactin‐releasing peptides (PrRPs), from the brain of the crucian carp. The deduced C‐RFa precursor has been shown to comprise 117 amino acids, encoding a single C‐RFa sequence. A comparative study of amino acid sequences has revealed that several sequences conserved in preproPrRPs are also found in the C‐RFa precursor. Furthermore, the abundant presence of the C‐RFa mRNA in the telencephalon, optic tectum, medulla oblongata, and proximal half eye ball was demonstrated by Southern blot analysis of RT‐PCR products.