Premium
Binding of neuronal ELAV‐like proteins to the uridine‐rich sequence in the 3′‐untranslated region of tumor necrosis factor‐α messenger RNA
Author(s) -
Sakai Koichiro,
Kitagawa Yoko,
Hirose Genjiro
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00206-9
Subject(s) - messenger rna , untranslated region , three prime untranslated region , rna binding protein , au rich element , microbiology and biotechnology , biology , tumor necrosis factor alpha , proinflammatory cytokine , gene expression , rna , post transcriptional regulation , gene , biochemistry , immunology , inflammation
Tumor necrosis factor‐α (TNF‐α) is a proinflammatory cytokine that is involved in the pathogenesis of several human CNS disorders. The AU‐rich element (ARE) in the 3′‐untranslated region (UTR) of TNF‐α mRNA is implicated in post‐transcriptional control of TNF‐α. In this study, we showed that a human neuronal ELAV‐like protein binds to the ARE in the 3′‐UTR of TNF‐α mRNA. The protein binds to the uridine stretch in AUUUA pentanucleotides inside the ARE in the 3′‐UTR of TNF‐α mRNA. The TNF‐α mRNA‐binding region in the protein appears to be identical to the c‐myc and IL‐3 mRNA‐binding regions. Moreover, this study showed that in vitro treatment of neuroblastoma cells with interleukin‐4 (IL‐4), which inhibits TNF‐α production, reduced the expression of the neuronal ELAV‐like proteins. These results suggest that the expression of neuronal ELAV‐like proteins may be closely associated with the expression of TNF‐α in neuronal cells.