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Activation of recombinant human SK4 channels by metal cations
Author(s) -
Cao Ying-Jun,
Houamed Khaled M.
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00194-5
Subject(s) - chemistry , recombinant dna , barium , strontium , metal , cadmium , potency , biophysics , biochemistry , inorganic chemistry , in vitro , organic chemistry , biology , gene
The effects of metal cations on the activation of recombinant human SK4 (also known as hIK1 or hKCa4) channels, expressed in HEK 293 cells, were tested using patch clamp recording. Of the nine metals tested, cobalt, iron, magnesium, and zinc did not activate the SK4 channels when applied, at concentrations up to 100 μM, to the inside of SK4 channel‐expressing membrane patches. Barium, cadmium, calcium, lead, and strontium activated SK4 channels in a concentration‐dependent manner. The rank order of potency was at Ca 2+ >Pb 2+ >Cd 2+ >Sr 2+ >Ba 2+ .