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In vivo operation of the pentose phosphate pathway in frog oocytes is limited by NADP + availability
Author(s) -
Preller Ana,
Guixé Victoria,
Ureta Tito
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00192-1
Subject(s) - pentose phosphate pathway , pentose , in vivo , fructose , intracellular , stimulation , glucose 6 phosphate , biochemistry , biology , glycolysis , chemistry , metabolism , enzyme , endocrinology , microbiology and biotechnology , fermentation
Evolution of CO 2 from labelled glucose microinjected into frog oocytes in vivo may be ascribed to the pentose‐P pathway, as measured by radioactive CO 2 production from [1‐ 14 C] and [6‐ 14 C]glucose. Coinjection of NADP + and [ 14 C]glucose significantly stimulated 14 CO 2 production. The effect depends on the amount of NADP + injected, half maximal stimulation being obtained at 0.13 mM. The increase in CO 2 production was also observed with microinjected glucose‐1‐P, glucose‐6‐P or fructose‐6‐P used as substrates. Phenazine methosulfate, mimicked the effects of NADP + . A high NADPH/NADP + ratio of 4.3 was found in the cells, the intracellular concentration of NADP + being 19 μM.