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Crocalbin: a new calcium‐binding protein that is also a binding protein for crotoxin, a neurotoxic phospholipase A 2
Author(s) -
Hseu Ming-Jhy,
Yen Chon-Ho,
Tzeng Mu-Chin
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00177-5
Subject(s) - peptide sequence , complementary dna , biochemistry , ef hand , calcium binding protein , binding protein , nucleic acid sequence , open reading frame , amino acid , cdna library , cyclic nucleotide binding domain , binding site , biology , chemistry , microbiology and biotechnology , calcium , gene , organic chemistry
Utilizing Marathon‐ready cDNA library and a gene‐specific primer corresponding to a partial amino acid sequence determined previously, the complete nucleotide sequence for the cDNA of crocalbin, which binds crotoxin (a phospholipase A 2 ) and Ca 2+ , was obtained by polymerase chain reaction. The open reading frame of the cDNA encodes a novel polypeptide of 315 amino acid residues, including a signal sequence of 19 residues. This protein contains six potential Ca 2+ ‐binding domains, one N ‐glycosylation site, and a large amount of acidic amino acid residues. The ability to bind Ca 2+ has been ascertained by calcium overlay experiment. Evidenced by sequence similarity in addition, it is concluded that crocalbin is a new member of the reticulocalbin family of calcium‐binding proteins.