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Stoichiometry of dipalmitoylphosphatidylcholine‐DNA interaction in the presence of Ca 2+ : a temperature‐scanning ultrasonic study
Author(s) -
Kharakoz D.P.,
Khusainova R.S.,
Gorelov A.V.,
Dawson K.A.
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00165-9
Subject(s) - dipalmitoylphosphatidylcholine , chemistry , dna , monolayer , molecule , stoichiometry , aqueous solution , crystallography , nucleotide , analytical chemistry (journal) , chromatography , biochemistry , organic chemistry , phospholipid , membrane , phosphatidylcholine , gene
DNA‐DPPC complexes can be prepared by means of a single step procedure of mixing DNA solution and aqueous lipid dispersion in the presence of calcium ions. Interaction between DPPC and DNA brings about a biphasic shape of melting curves corresponding to the free lipid and the strongly bound one. The amount of the strongly bound lipid is 5 molecules per nucleotide which is close to the size of the first lipid monolayer around DNA molecule.