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Isolation of a highly active PSII‐LHCII supercomplex from thylakoid membranes by a direct method
Author(s) -
Eshaghi Said,
Andersson Bertil,
Barber James
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00149-0
Subject(s) - thylakoid , chemistry , isolation (microbiology) , biophysics , photosynthesis , biochemistry , biology , chloroplast , microbiology and biotechnology , gene
We have developed a simple and novel method to isolate a highly pure and active photosystem (PS) II complex, directly from thylakoid membranes. This complex is a discrete particle and contains all the proteins of the oxygen evolving complex and a set of chlorophyll a/b binding proteins. The intactness of both the donor side and the acceptor side has resulted in a very high oxygen evolution activity and therefore offers a superior experimental system to that of PSII enriched membrane fragments in which there is heterogeneity in activities and biochemical composition.