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Increased oxidative stress in the RAW 264.7 macrophage cell line is partially mediated via the S‐nitrosothiol‐induced inhibition of glutathione reductase
Author(s) -
Butzer Urwe,
Weidenbach Hans,
Gansauge Susanne,
Gansauge Frank,
Beger Hans G,
Nussler Andreas K
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00139-8
Subject(s) - glutathione , glutathione reductase , oxidative stress , chemistry , nitric oxide , biochemistry , superoxide , enzyme , glutathione disulfide , reductase , microbiology and biotechnology , biology , glutathione peroxidase , organic chemistry
We investigated whether endogenously or exogenously produced nitric oxide (NO) can inhibit cellular glutathione reductase (GR) via the formation of S‐nitrosothiols to decrease cellular glutathione (GSH) and increase oxidative stress in RAW 264.7 cells. The specificity of this inhibition was demonstrated by addition of a NO‐synthase inhibitor, and met‐ or oxyhemoglobin. Using isolated GR we found that only certain NO donors inhibit this enzyme via S‐nitrosothiol. Furthermore, we found that cellular GSH decrease is paralleled by an increase of superoxide anion production. Our results show that the GR enzyme is a potential target of S‐nitrosothiols to decrease cellular GSH levels and to induce oxidative stress in macrophages.