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Carboxyatractyloside increases the effect of oleate on mitochondrial permeability transition
Author(s) -
Chávez Edmundo,
Zazueta Cecilia,
Garcı́a Noemı́
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00128-3
Subject(s) - mitochondrial permeability transition pore , mitochondrion , transmembrane protein , translocase , biophysics , inner mitochondrial membrane , chemistry , transition (genetics) , nucleotide , biology , biochemistry , apoptosis , chromosomal translocation , receptor , programmed cell death , gene
Addition of a low concentration of carboxyatractyloside (0.075 μM) renders mitochondria susceptible to the opening of the non‐specific pore by 5 μM oleate, in a cyclosporin A‐sensitive fashion. Matrix Ca 2+ efflux as well as collapse of the transmembrane potential reveal permeability transition. The effect of oleate is reached after the titration, by carboxyatractyloside, of 38 pmol of adenine nucleotide translocase per mg mitochondrial protein. We propose that permeability transition may result from an additive action of carboxyatractyloside plus oleate on the ADP/ATP carrier.