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Identification of a ribonuclease H gene in both Mycoplasma genitalium and Mycoplasma pneumoniae by a new method for exhaustive identification of ORFs in the complete genome sequences
Author(s) -
Bellgard Matthew I,
Gojobori Takashi
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00075-7
Subject(s) - mycoplasma genitalium , orfs , identification (biology) , mycoplasma pneumoniae , genome , ribonuclease , gene , genetics , biology , mycoplasma , mycoplasma gallisepticum , computational biology , mycoplasmataceae , microbiology and biotechnology , virology , mollicutes , peptide sequence , open reading frame , rna , pneumonia , medicine , botany , chlamydia trachomatis
Exhaustive identification of open reading frames in complete genome sequences is a difficult task. It is possible that important genes are missed. In our efforts to reanalyze the intergenic regions of Mycoplasma genitalium and Mycoplasma pneumoniae , we have newly identified a number of new open reading frames (ORFs) in both M. genitalium and M. pneumoniae . The most significant identification was that of a ribonuclease H enzyme in both species which until now has not been identified or assumed absent and interpreted as such. In this paper we discuss the biological importance of RNase H and its evolutionary implication. We also stress the usefulness of our method for identifying new ORFs by reanalyzing intergenic regions of existing ORFs in complete genome sequences.