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Concatemerization of tRNA molecules in the presence of trivaline derivative
Author(s) -
Streltsov Sergey A.,
Martinkina Larissa P.,
Vengerov Yuri Yu.
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00072-1
Subject(s) - transfer rna , molecule , uranyl acetate , chemistry , electron microscope , fluorescence microscope , crystallography , fluorescence , biophysics , stereochemistry , biochemistry , rna , organic chemistry , biology , physics , quantum mechanics , optics , gene
The interaction of tRNA with trivaline dansyl hydrazide trifluoroacetate (DHTV) has been studied. The shape of curves of fluorimetric titration of tRNA with DHTV and vice versa can be explained only by formation of DHTV dimers on tRNA molecules, and subsequent association of DHTV‐saturated tRNA molecules with each other. The ability of tRNA molecules to form concatemers in solution in the presence of DHTV has been demonstrated by electron microscopy. Electron microscopy of the tRNA‐DHTV complexes stained with uranyl acetate revealed flexible rods 6–7 nm thick and up to several micrometers long.