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The pro‐phenoloxidase of coleopteran insect, Tenebrio molitor , larvae was activated during cell clump/cell adhesion of insect cellular defense reactions 1
Author(s) -
Lee Hyun Seong,
Cho Mi Young,
Lee Kwang Moon,
Kwon Tae Hyuk,
Homma Ko-ichi,
Natori Shunji,
Lee Bok Luel
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00067-8
Subject(s) - biology , hemolymph , insect , cell adhesion , adhesion , microbiology and biotechnology , cell , bombycidae , clone (java method) , larva , biochemistry , bombyx mori , botany , gene , chemistry , organic chemistry
To characterize the proteins involved in cell clump/cell adhesion of insect cellular defense reactions, we induced the cell clump/cell adhesion reaction in vitro with the hemolymph of larvae of the coleopteran insect, Tenebrio molitor . The 72 kDa protein was specifically enriched in the residues of cell clump/cell adhesion and was purified to homogeneity. A cDNA clone for the 72 kDa protein was isolated. We found that the 72 kDa protein was an activated phenoloxidase from Tenebrio pro‐phenoloxidase. We suggest that activated phenoloxidase is involved in the cell clump/cell adhesion reaction as well as in the synthesis of melanin.