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Growth hormone induces insulin‐like growth factor‐I gene transcription by a synergistic action of STAT5 and HNF‐1α
Author(s) -
Metón Isidoro,
Boot Elmieke P.J.,
Sussenbach John S.,
Steenbergh Paul H.
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00064-2
Subject(s) - transfection , transcription factor , stat5 , growth factor , transcription (linguistics) , growth hormone , promoter , luciferase , cell culture , microbiology and biotechnology , biology , chemistry , reporter gene , gene , gene expression , receptor , endocrinology , hormone , signal transduction , biochemistry , genetics , linguistics , philosophy
Salmon insulin‐like growth factor‐I (sIGF‐I) expression is, as in mammals, induced by growth hormone (GH). To elucidate the mechanism by which GH stimulates the transcription of the IGF‐I gene, we transiently transfected Hep3B cells expressing the rat GH receptor with a sIGF‐I promoter‐luciferase reporter construct. Activation of the construct by GH added to the medium of the transfected cells was observed when two specific transcription factors, STAT5 and HNF‐1α, were simultaneously overexpressed in these cells. This finding demonstrates for the first time a GH‐dependent activation of an IGF‐I promoter construct in an immortalized laboratory cell line.