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Hybridization of antisense oligonucleotides with the 3′ part of tRNA Phe
Author(s) -
Petyuk Vladislav A.,
Zenkova Marina A.,
Giege Richard,
Vlassov Valentin V.
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00063-0
Subject(s) - oligonucleotide , transfer rna , chemistry , microbiology and biotechnology , biochemistry , biology , rna , gene
The interaction of antisense oligodeoxyribonucleotides with yeast tRNA Phe was investigated. 14–15‐mers complementary to the 3′‐terminal sequence including the ACCA end bind to the tRNA under physiological conditions. At low oligonucleotide concentrations the binding occurs at the unique complementary site. At higher oligonucleotide concentrations, the second oligonucleotide molecule binds to the complex due to non‐perfect duplex formation in the T‐loop stabilized by stacking between the two bound oligonucleotides. In these complexes the acceptor stem is open and the 5′‐terminal sequence of the tRNA is accessible for binding of a complementary oligonucleotide. The results prove that the efficient binding of oligonucleotides to the 3′‐terminal sequence of the tRNA occurs through initial binding to the single‐stranded sequence ACCA followed by invasion in the acceptor stem and strand displacement.