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Cell‐free immunology: construction and in vitro expression of a PCR‐based library encoding a single‐chain antibody repertoire
Author(s) -
Makeyev Eugeny V.,
Kolb Vyacheslav A.,
Spirin Alexander S.
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00055-1
Subject(s) - repertoire , cloning (programming) , computational biology , antibody repertoire , antibody , microbiology and biotechnology , in vitro , biology , single chain , computer science , immunology , genetics , physics , acoustics , programming language
A novel cloning‐independent strategy has been developed to generate a combinatorial library of PCR fragments encoding a murine single‐chain antibody repertoire and express it directly in a cell‐free system. The new approach provides an effective alternative to the techniques involving in vivo procedures of preparation and handling large libraries of antibodies. The possible use of the described strategy in the ribosome display is discussed.