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Localization of curved DNA and its association with nucleosome phasing in the promoter region of the human estrogen receptor α gene
Author(s) -
Wada-Kiyama Yuko,
Kuwabara Kentaro,
Sakuma Yasuo,
Onishi Yoshiaki,
Trifonov Edward N,
Kiyama Ryoiti
Publication year - 1999
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(99)00041-1
Subject(s) - nucleosome , microbiology and biotechnology , dna , promoter , hormone response element , biology , estrogen receptor , gene , base pair , chemistry , genetics , histone , gene expression , cancer , breast cancer
We determined DNA bend sites in the promoter region of the human estrogen receptor (ER) gene by the circular permutation assay. A total of five sites (ERB−4 to −1, and ERB+1) mapped in the 3 kb region showed an average distance of 688 bp. Most of the sites were accompanied by short poly(dA)·poly(dT) tracts including the potential bend core sequence A 2 N 8 A 2 N 8 A 2 (A/A/A). Fine mapping of the ERB−2 site indicated that this A/A/A and the 20 bp immediate flanking sequence containing one half of the estrogen response element were the sites of DNA curvature. All of the experimentally mapped bend sites corresponded to the positions of DNA curvature as well as to nucleosomes predicted by computer analysis. In vitro nucleosome mapping at ERB−2 revealed that the bend center was located 10–30 bp from the experimental and predicted nucleosome dyad axes.